Reena Singh

6 Questions 5 Answers 0 Followers

Questions related from Reena Singh

Has anyone tried to use artificial miRNA in mammalian cells?

Can anyone share their experience with artificial miRNA in mammalian cells. I am wondering, if one can use them in for example, U2OS cells to simultaneously silence all the isoforms of a...

07 July 2017 248 0 View

Is anyone has experience of transfecting hESC derived cardiomycytes? Which transfection reagent or methods can be used to transfect cardiomyocytes?

I would like to transfect human embryonic stem cell differentiated cardiomyocytes with GFP plasmid. Based on the literature, it does seems like, these cells are relatively difficult to transfect....

09 September 2016 6,056 4 View

Has anyone used Lipofectamine 3000 transfecting reagent to transfect H9 (human embryonic stem cells) cultured in mTeSR media?

I would like to use Lipofectamine 3000 (Life technologies) to transfect human embryonic stem cells (H9) and make the selection by sorting with FACS aria II. My basic concerns includes - Whether, I...

06 June 2016 2,465 2 View

What concentration of bleomycin one can use to induce ds DNA damage in HEK293 cells?

Further, how can you prepare concentrations of bleomycin? Can somebody suggest to me how exactly I can prepare bleomycin stock solution and what is the most efficient and appropriate...

02 February 2014 6,016 3 View

What is the correlation between DNA damage and the development of malignant tumors in cancer?

As DNA damages are mainly involved in the formation of primary tumor but somehow it diverts towards the development of melignant tumor, which leads to metastasis property. What is that mechanism...

01 January 2014 5,913 8 View

Is it possible to keep plates with fixed cells in half of the wells in PBS and live cell in other half with culture medium?

I am doing differentiation studies with hES cells. I have a whole experiment on one plate (no coverslips) but different time points to fix cells in certain wells for immunofluorescence staining....

01 January 1970 5,230 1 View