Is it possible to keep plates with fixed cells in half of the wells in PBS and live cell in other half with culture medium?

01 January 1970 1 5K Report

I am doing differentiation studies with hES cells. I have a whole experiment on one plate (no coverslips) but different time points to fix cells in certain wells for immunofluorescence staining. Is it possible to fix the cells with 4% PFA or 95% methanol and keep it in PBS while time course running for other cells in 37 degrees in incubator ?

Many Thanks for your response in advance, Reena

Christoph-Rüdiger von Bredow

Dear Reena,

you have take care that no formaldehyde or methanol vapours will reach the living cells since both chemicals may have effects on the cells. The only way I can imagine to do so is to culture the cells in separate dishes.

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