I want to extract genomic DNA from tumour cells. Can anyone help me how? most simple without PCI.
Could you specify - How long would you like the fragments to be? Thanks.
You can use qiagen genomic DNA extraction kit, they give full and very easy protocol.
good luck
If you have 5 to 10 million cells DNAzol is an option.
I would not use it if you had less cells.
you can use Trizol.
Thanks everyone for your valuable information!
Kindly help to interpret GC-MS of plant extract? how to relate a particular compound from the spectra. What is important point to consider? Thank you all
09 October 2018 7,494 5 View
Dear researcher, I would like to identify and confirm plants by molecular method. So, what gene will be best suited for this purpose?
08 September 2018 5,345 0 View
Dear Experts, I would to carry out a research on Plant source medicinal or ethno-pharmaceutical in vitro and in vivo. I would to focus on Biochemical-Physiological profiling and molecular...
06 July 2018 7,714 0 View
Can cancer cells from animal origin growth, develop and proliferate in human? What if not, what exactly is the reason? is it immune system incompatible alone for not proliferation? Thanks
07 August 2016 6,523 1 View
Can ulcer be induced and treated well in hours? As far my understanding is concern, it is chronic symptom. Does the healing of ulcer involves division of cell or tissue formation? Thanks
07 August 2016 1,893 1 View
Dear researchers, I need to perform biochemical experiment data to analysis statistical significance. I have more two groups for all experiments. But I am not sure what statistical parameter will...
03 April 2016 1,982 3 View
Respected Researchers, I would like to know what is the principle behind the Western blotting? How will be the expression of interest protein if treated cancer cell with drugs? I read many...
03 April 2016 3,939 2 View
Researchers, can anyone suggest me western blotting technique? I need to perform western blotting but i have no practical experienced yet. How to develop the band or expression of protein on...
02 March 2016 3,647 6 View
Dear Researchers, I need to analyse biochemical experiments and i am confused which parameters will i use. 1. I performed in vivo and in vitro antioxidant enzyme activity of different treatment...
02 March 2016 1,272 6 View
I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate. Can anyone share me...
01 February 2016 6,760 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View