Hi all,
I am trying to measure synaptic transmission in cultured neurons. I plan to use WGA-Cre in postsynaptic side to trigger the gene expression in presynaptic neurons. However, I could not find papers using WGA-Cre in cultured neuron with this purpose. Could you recommend some? Most papers used it in vivo with AAV injection into the brain. Or, is there some reasons that WGA-Cre can not used in vitro?
Any suggestion will be appreciated.
Thanks a lot.
Yu