11 Questions 36 Answers 0 Followers
Questions related from Chenglong Yu
Hi all, I am working on live-imaging of cultured neurons. I has a question about electrical stimulation. Is it comparable for the following protocols: 1. 50 Hz for 6 s; 2. 10 Hz for 3 s; They all...
31 July 2018 9,982 3 View
Hi all, I am trying to measure synaptic transmission in cultured neurons. I plan to use WGA-Cre in postsynaptic side to trigger the gene expression in presynaptic neurons. However, I could not...
15 June 2017 6,048 0 View
Hi all, I want to measure Ca2+ influx through NMDAR. Is it fine to do Ca2+ imaging with TTX, Mg2+ free ACSF and add NMDA? Do I need to block voltage-gated Ca2+ channels (VGCCs) at the same...
01 March 2017 2,762 10 View
Hi, I am working of P0 neuron culture and imaging. Recently, I found someone said that there are no functional NMDAR in P0 mice culture. They said that some receptors lost their function in P0...
14 February 2017 2,080 6 View
Hi guys, I find some abnormal increase of Ca2+ influx induced by electrical stimulation in my cultured neurons. I want to clamp it to normal level by BAPTA-AM, which is membrane permeable. Does...
10 October 2016 8,209 5 View
Hi guys, I am working on live-cell imaging of primary cultured neurons. I want to identify the formation of synapses in my live-imaging. I doubt the transfection of synaptic markers as they may go...
04 July 2016 4,626 6 View
Hi, I am looking for good fluorescent markers to identify pre-/postsynaptic sites. Something like PSD95-GFP and synaptophysin-mCherry (Syp–Cherry). Any suggestions will be appreciated. Besides,...
17 February 2016 4,623 0 View
Hi guys, I am looking for a binding partner for my molecule. It should be a new one as no one knows what it is. I feel that there should have a binding partner. But it is just a kind of...
16 December 2015 8,889 6 View
Hell guys, I am working on synaptic release triggered by field stimulation. We use the parallel platinum electrode. I can see some release using syt-pHluorin. But somehow my labmate could not see...
10 August 2015 6,086 16 View
Hi, I am a new student who is going to do some electroporation on primary cortical neurons. Once harvesting cortical neurons, how much for amplitude and duration for the pulse shall I use? I am...
03 July 2015 1,087 23 View
I would like to quantify the expression of a protein in neurons. However, it is also highly expressed in glia. How can I exclude the glia protein expression? I mean to test the protein levels...
12 March 2015 681 4 View
