7 Questions 18 Answers 0 Followers
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Hi all, I am working on live-imaging of cultured neurons. I has a question about electrical stimulation. Is it comparable for the following protocols: 1. 50 Hz for 6 s; 2. 10 Hz for 3 s; They all...
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Hi guys, I find some abnormal increase of Ca2+ influx induced by electrical stimulation in my cultured neurons. I want to clamp it to normal level by BAPTA-AM, which is membrane permeable. Does...
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Hi, I am a new student who is going to do some electroporation on primary cortical neurons. Once harvesting cortical neurons, how much for amplitude and duration for the pulse shall I use? I am...
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I would like to quantify the expression of a protein in neurons. However, it is also highly expressed in glia. How can I exclude the glia protein expression? I mean to test the protein levels...
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