When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

Hi. Can fish parasitic crustaceans also be used to clean up pollution sites?

A number of research have talked about parasitic crustaceans as bioindicators of pollution, however, there has not been mention of the use of these organisms in cleaning up the metals and/or...

04 May 2019 6,041 1 View

Is this a Porcellidium copepod?

I found these individuals in meiofaunal samples associated to intertidal coralline turfs. Do you think they belong to the genus Porcellidium?

04 February 2019 8,239 5 View

Is this a Porcellidium copepod?

I found these individuals in meiofaunal samples associated to intertidal coralline turfs. Do you think they belong to the genus Porcellidium?

01 February 2019 2,740 6 View

Smallest eukaryote 16S rRNA?

We have just sequenced the mitochondrial genome of a large freshwater copepod, and found that its 16S rRNA is unusually short (527 bp). Is anyone aware of a shorter eukaryote 16S, or can anyone...

08 September 2018 1,698 8 View

Smallest eukaryote 16S rRNA?

We have just sequenced the mitochondrial genome of a large freshwater copepod, and found that its 16S rRNA is unusually short (527 bp). Is anyone aware of a shorter eukaryote 16S, or can anyone...

02 September 2018 2,555 8 View

Could someone provide me the PDFs of these references?

Could someone provide me the PDFs of these references? 1) J.B.J. Wells. 2007. An annotated checklist and keys to the species of Copepoda Harpacticoida (Crustacea). Zootaxa . 1568: 1-872. 2)A....

06 July 2018 5,220 1 View

Can somebody please help me identify a parasitic copepod found in the gut of the abalone Haliotis asinina?

Can somebody please help me identify a parasitic copepod found in the gut of the abalone Haliotis asinina? Please see the photos in the attachment. Your favorable response is highly appreciated....

03 April 2018 376 2 View

Zooplankton biovolume conversion factors?

11 December 2017 9,709 7 View

The biology of Calanoid copepods, Machline

Does anybody have a copy of the book The biology of Calanoid copepods, Machline?? I'll appreciate any help!!!

07 August 2017 8,977 3 View

I want to ask what area or technique should I use in image processing to identify copepods down to species level(my subject)?

I am a BS biology graduate and I'm taking up a second course major in computer science. So my research is about image processing used to identify copepods as my subject. but I don't know which...

02 March 2017 2,975 0 View