David A Johnson

6 Questions 8 Answers 0 Followers

Questions related from David A Johnson

MRNA de novo assembly: How good is Geneious de novo assembly of transcriptome data using their assembly function (or other suggestions for assembly)?

I am trying assemble transcriptome data from a Tubificinae annelid, genus Limnodrilus. I sent in tissue and eukaryotic RNA seq was run successfully (poly-A tail RNAs). However, the genomes I...

11 July 2023 3,827 3 View

Is a copepod (or an amoebozoa) with <82% sequence identity for the COI gene with any known copepod (or amoeba) something to be further investigated?

I have been working on a unique freshwater habitat and, from environmental sequencing, found copepod COI DNA that is quite divergent from published sequences (

10 March 2023 9,791 0 View

Can I use a Taq mastermix for A-tailing?

I have gel-purified PCR product from a Q5 high-fidelity amplification to which I want to add the A tails for TOPO-TA cloning. The protocols I find all say to use Taq polymerase and dATP, but can I...

25 September 2019 1,050 3 View

Is anyone using Hivebench? Is it down?

I just started using Hivebench as an electronic lab notebook and was liking it but Tuesday, June 18, 2019, it stopped all updating, syncing, and I couldn't access it online page. It's server seems...

19 June 2019 6,325 1 View

Does our preliminary COI sequence data suggest a new planarian? Where to find interested planarian experts?

We have discovered what could be a novel planarian in a unique, isolated aquatic environment. Preliminary COI sequencing and a BLAST search reveals only about 81% similarity to anything published....

13 March 2019 3,927 3 View

What are these bristles on the kudzu bug?

Can anyone tell me what this pair of bristles found associated with every spiracle in the kudzu bug are? A pair is always found near the spiracle and the "crater" around the base of the bristle...

21 November 2015 4,462 6 View