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Questions related from David A Johnson
In the course of exploring cDNA data from an annelid transcriptome, I have come across what looks like a dicistronic mRNA. I am now verifying that the two CDSs that seem to be on the same mRNA...
09 December 2024 740 0 View
Here’s my dilemma. I have run out of a rare gDNA sample that was donated to me. So far, I’ve been unable to locate any specimens of that very rare organism from which I need to amplify one...
12 November 2024 9,174 4 View
As you will be able to tell, I am a bit of a novice in this field. I have the cDNA sequence of the glutathione reductase gene from a transcriptome of a tubificine worm I am studying. I was able to...
16 August 2024 1,237 2 View
I am trying assemble transcriptome data from a Tubificinae annelid, genus Limnodrilus. I sent in tissue and eukaryotic RNA seq was run successfully (poly-A tail RNAs). However, the genomes I...
11 July 2023 3,881 3 View
I have been working on a unique freshwater habitat and, from environmental sequencing, found copepod COI DNA that is quite divergent from published sequences (
10 March 2023 9,828 0 View
The rRNA genes present in eukaryotes are usually maintained as completely identical copies, but cases have been found where an individual organism has variation in 18S, 5.8S, or 28S rDNA...
13 March 2022 4,240 0 View
With limited access to my physical files, I am searching for a publication I read that suggest that sulfur tolerance may have been a trait of all annelids that has been lost by most modern worms....
02 August 2020 8,621 0 View
Can someone get me started on how to construct a single phylogenetic tree from multiple alignments of three mitochondrial gene sequences (12S, 16S, and COI)? I am trying to establish the...
26 October 2019 1,152 4 View
I have gel-purified PCR product from a Q5 high-fidelity amplification to which I want to add the A tails for TOPO-TA cloning. The protocols I find all say to use Taq polymerase and dATP, but can I...
25 September 2019 1,085 3 View
I just started using Hivebench as an electronic lab notebook and was liking it but Tuesday, June 18, 2019, it stopped all updating, syncing, and I couldn't access it online page. It's server seems...
19 June 2019 6,366 1 View
I want to store gel-sized aliquots of melted agarose at 70° C, capped to prevent evaporation. Is this possible? How long would it be stable?
28 March 2019 7,273 0 View
We have discovered what could be a novel planarian in a unique, isolated aquatic environment. Preliminary COI sequencing and a BLAST search reveals only about 81% similarity to anything published....
13 March 2019 3,978 3 View
Can anyone tell me what this pair of bristles found associated with every spiracle in the kudzu bug are? A pair is always found near the spiracle and the "crater" around the base of the bristle...
21 November 2015 4,511 6 View
