Currently, I am using commercial available human serum from Sigma (Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered). However, most of the papers I found used human serum from donor. The concentration I use is 2% instead of 10% as substitute for FBS. Is there anyone has experience or know any published paper that use the same human serum (from Sigma) for MSC culture?
Hello
Kindly look at link in below
http://www.biomedsearch.com/attachments/00/24/19/85/24198529/sccaa-4-039.pdf
Good Luck
In my opinion and experience, 2% serum is not sufficient to culture human Wharton's jelly derived MSC. It is hence, advisable to use at least 10% serum. For a better health of the cells, growing of cells in 15-20% serum is also recommended.
In my experience 5% es the minimum percent for human cell culture that could be used, but it still low. If you can increase the percenteeven until 15%it would be better.
in the other hand, you can use inactivated plasma from donors, its a simple procedure and works better, but any way, human serum from sigma should work.
Good luck with your experiments.
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