Nur Afiqah Mohamad

4 Questions 6 Answers 0 Followers

Questions related from Nur Afiqah Mohamad

Can I use DMEM to reconstituted bFGF?

Instead of using PBS or sterile water, I use DMEM to prepare bFGF. Is it would be stable if it is prepared in DMEM/culture media? Does anyone have any ideas or information?

07 August 2018 6,882 4 View

Can cells forgotten in -20 degree for 48hours be used?

I was freezing Wharton Jelly stem cell in Mr.Frosty and forgot them in -20 degree for 48hours (due to weekend). I transferred them to -80 degree in Monday morning. Does anyone has experienced this?

30 July 2018 5,728 10 View

Cryopreserved mesenchymal stem cells are viable but part of them loses their attachment capacity. Anyone knows why?

I revived cells from LN2 storage but the cells don't want to attach.

29 May 2018 4,957 5 View

Is 2% of human serum enough/sufficient for MSC culture derived from Wharton's Jelly?

Currently, I am using commercial available human serum from Sigma (Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered). However, most of the papers I found used human serum...

01 January 1970 6,906 3 View