01 January 1970 3 5K Report

Iodin as a liquid form proved it is effectiveness against SARS COV2 infection, so how can the dentist use it in clinical manner with minimal side effects?

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The future of stem cells in brain repair??

The future of stem cells in brain repair and TBI???

08 May 2024 8,126 0 View

Why do I get different results between Coomassie Gels and Western Blot?

Hi everyone, I hope you're doing well. I've encountered an interesting phenomenon in my research and would appreciate your insights. When running gels and staining them with Coomassie, I'm...

18 March 2024 3,416 0 View

Why am I unable to detect pERK in HEK293T cells through Western Blotting?

Greetings to all, I have attempted to detect pERK using western blotting with HEK 293T cells, but I am unable to do so. I overexpressed a gene that caused pERK to be overproduced, and I was...

21 December 2023 7,195 3 View

Inner geometry suppression when refining the mesh?

Dear ICEM CFD users, Could you please help me to solve this problem? I have a inner geometry inside an other. A coarse mesh of such a configuration captures the inner geometry but a refined mesh...

25 September 2023 2,887 0 View

Do conifers produce tracheid with spiral thickness? If not, Is tracheids spiral thickness the same thing as the "Cellulose Microfibril Angle"?

Hello, Tracheid in Cupressaceae and other conifers may exhibit ornamentation on their walls that resemble helical spiral thickness little bit, as result of an angle in Microfibrils. What is the...

22 January 2023 5,334 2 View

How can i do Mycoplasma test in tissues ?

is it possible to do mycoplasma contamination test in my Warthon jelly tissues frozen at -80°C ? all what i found n the internet is compatible with cell culture supernatant and nothing about...

30 November 2022 6,320 3 View

How do I interpret my MTT assay results, and what statistical tests should I do for cell viability?

I have completed an experiment where I exposed rainbow trout liver cells to copper concentrations of 0.01, 0.1, 1.0 M, along with a negative control. I have recorded the cell viability % at each...

20 March 2021 9,445 7 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 515 6 View

I need a help from a spring technician or some who know a lot about heavy duty spring ?

I need a spring technician or some who know a lot about heavy duty spring to ask him/her a couple of questions in my bachelor thesis. The question will be in direction of choosing the right...

20 December 2020 8,646 0 View

What happens to a gas after compressing it for 10 years?

Will it lose a fraction of its expansion power assuming it was insulated in this system? Because I am designing an insulated gas spring. what I want to know if the gas will lose some of its...

07 December 2020 6,363 3 View

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Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

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Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

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How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

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Where is this method for determining the content of H2S in drinking water in the field?

Take 70 mL of water from the source (under a stream) in a beaker, pour into an Erlenmeyer flask, add 2 mL of starch solution and immediately titrate with iodine solution in KJ until a blue color...

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Is distance education effective as Onsite education?

Since the experience of COVID-19 in the world, educational bodies, institute of higher learning has adopted distance education. Even though the use has existed ever before the COVID-19 issue....

22 May 2024 7,430 3 View

Why the clear zone indicating amylase activity on agar plates dissappears after some hours?

I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...

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Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

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What are Trends in viral infection among cancer patients?

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