Hallo everyone!
My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines. However, no one in the lab ever worked with this way of the DNA-administration to animals.
There's a lot of information on the topic in the public papers, however I would like to hear from a person that has a personal expertize in intaranasal airborn DNA-delivery.
My questions:
- What is the best-working transfection reagent, protocol or chemistry (in your personal experience)?
- Will PEI or lipofectamine work well or there is a definite need to swith to a different transfection reagents, to mix with plasmid DNA before applying to mice i.n.?
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Dear Alexandr,
we did a few experiments with in vivo-JetPEI from Polyplus. It's a specific PEI for in vivo use. It has also been used in clinical trials. Given intranasaly, it provoked significant cellular and humoral responses. However, it was less immunogenic than several other vaccine platforms we used, like viral vectors for example, and had in our hands more unwanted inflammatory side effects.