Alexandr V Shustov

8 Questions 10 Answers 0 Followers

Questions related from Alexandr V Shustov

Growing poorly attaching but adhesion-dependent cells in roller bottles?

Greetings to all colleagues! Please persons who have experience of working will the cell line HEK293FT. Exactly "FT" not just 293T. The HEK293FT is used for packaging lentiviral vectors and this...

03 February 2025 2,976 0 View

How to control adhesion cell density in large-diameter roller bottles?

Good day/evening for everyone! I have a technical questions, about microscopy of roller bottles, and a more broad problem: how to control adhesion-dependent cells in large roller bottles? 1) My...

25 December 2024 3,723 0 View

A protein or its multimer undergoes a proteolytic processing - Need to know is multimerization required for the processing?

Hallo everyone! Need an advice, in an experimental setup. In brief, I study a protein which undergoes a proteolytic processing. It is known that the protein is present upon physiological...

14 April 2024 1,954 1 View

Why capping reagents must be dry?

Would anyone kindly explain, why during oligosynthesis I have to use dry capping reagents CapA and CapB? In many manuals it is mandated that all reagents for oligosynthesis must be water-free,...

21 March 2022 2,653 3 View

Oligosynthesis: can I add P2O5 to the activator solution to dry out possible residual moisture?

Problem: Our lab has a DNA synthesizer and sometimes I make in-home synthesized oligonucleotides. From a certain time I have a problem with synthesis of long (>40 nt) oligonucleotides. Short...

22 January 2022 1,161 7 View

How can I measure a small-titers viremia if the nonspecific plasma toxicity is present in my experiments?

Hallo everyone, I have a problem with plasma toxicity in cell cultures during virus titrations. More specifically, we infect CD-1 mice with a virus, which we believe is capable of causing a...

05 February 2021 9,678 0 View

Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

21 January 2021 8,524 2 View

A virus (SARS-CoV-2) does not kill all cells in a monolayer, why?

Hallo all, I am engaged in a project which involves growing the SARS-CoV-2 virus in cell cultures. We use Vero E6 cells to amplify SARS-CoV-2. The idea is to produce a collection of viral...

16 July 2020 9,090 36 View