Alexandr V Shustov

6 Questions 10 Answers 0 Followers

Questions related from Alexandr V Shustov

A protein or its multimer undergoes a proteolytic processing - Need to know is multimerization required for the processing?

Hallo everyone! Need an advice, in an experimental setup. In brief, I study a protein which undergoes a proteolytic processing. It is known that the protein is present upon physiological...

14 April 2024 1,667 1 View

Why capping reagents must be dry?

Would anyone kindly explain, why during oligosynthesis I have to use dry capping reagents CapA and CapB? In many manuals it is mandated that all reagents for oligosynthesis must be water-free,...

21 March 2022 2,593 3 View

Oligosynthesis: can I add P2O5 to the activator solution to dry out possible residual moisture?

Problem: Our lab has a DNA synthesizer and sometimes I make in-home synthesized oligonucleotides. From a certain time I have a problem with synthesis of long (>40 nt) oligonucleotides. Short...

22 January 2022 1,105 7 View

How can I measure a small-titers viremia if the nonspecific plasma toxicity is present in my experiments?

Hallo everyone, I have a problem with plasma toxicity in cell cultures during virus titrations. More specifically, we infect CD-1 mice with a virus, which we believe is capable of causing a...

05 February 2021 9,617 0 View

Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

21 January 2021 8,337 2 View

A virus (SARS-CoV-2) does not kill all cells in a monolayer, why?

Hallo all, I am engaged in a project which involves growing the SARS-CoV-2 virus in cell cultures. We use Vero E6 cells to amplify SARS-CoV-2. The idea is to produce a collection of viral...

16 July 2020 9,041 36 View