Dear colleagues, I am searching for a fish cell line to study the natural extracts' immunostimulant activity. Could you please provide me with information about the best model to use, and how to obtain the cell line? Cell lines from other fish tissues are also welcome.
Hi Dear..
There are several fish cell lines that have been used to study the immunostimulant activity of natural extracts. One commonly used fish cell line is the RTG-2 rainbow trout cell line, which is derived from the head kidney of a rainbow trout and is a commonly used model for studying the immune response in fish. Other commonly used fish cell lines include the CHSE-214 and CHSE-255 cell lines, which are derived from the head kidney of Atlantic salmon, and the PLHC-1 cell line, which is derived from the liver of a goldfish.
To obtain these fish cell lines, you can contact organizations such as the American Type Culture Collection (ATCC) or the European Collection of Cell Cultures (ECACC). They maintain a wide variety of fish cell lines, including those listed above. These organizations generally require that you sign a Material Transfer Agreement (MTA) in order to obtain the cells. This is a legal agreement that outlines the conditions under which the cells can be used, as well as any restrictions on their distribution. Some institutions like universities, also keep their own fish cell lines that is used by researchers. You can contact them directly and see if they have the cell line you are looking for and see if they have a sharing program.
It is also important to keep in mind that working with cell lines can have its own set of risks, such as potential contamination, genetic instability and also they are not a perfect representation of the real in vivo scenario. Additionally, Fish cell lines are relatively scarce and are not well-characterized as mammalian cell lines, so it is important to confirm the identity and authenticity of the cell line prior to use, for example by karyotype analysis or genotyping, which can be done by institutions which keep the cell lines or by commercial providers.
I hope this information is helpful!
There are a number of fish cell lines that have been used to study the immune response, including the rainbow trout head kidney (RTHK) cell line, the common carp head kidney (CCHK) cell line, and the tilapia head kidney (THK) cell line. These cell lines have been found to be suitable models for studying the immune response to pathogens and other immunostimulants.
In terms of obtaining these cell lines, they are often available from commercial sources such as the American Type Culture Collection (ATCC), which provides a wide range of fish cell lines for research. Additionally, some research institutions may also have fish cell lines available for distribution. It is worth noting that the availability of these cell lines may vary based on location, so it is best to check with a local supplier or contact a researcher that works with the specific fish cell line you are interested in.
Overall, the best model to use will depend on the specific research question you are trying to answer, and the type of fish you are interested in studying. You may want to consult with a fish immunologist or a researcher that has experience working with fish cell lines to determine which model would be most appropriate for your study.
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