I want to see the cellular effect of my compounds found via high throughput screening. I am using MEF for my cell-based assays.
The compounds I have dissolved in DMSO (synthesis grade) https://www.carlroth.com/de/de/loesungsmittel-zur-synthese/dimethylsulfoxid-%28dmso%29/p/7029.1
Can these compounds be used in cellular assays? given the fact that they are not in cell culture grade DMSO. From people´s experience here, how much DMSO can the cells tolerate?
PS - Earlier I didn't know about dissolving them in cell culture grade DMSO.
Thanks,
Saif