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Questions related from Mohd Saif
via HTS I found Inhibitors (IC50 between 1 to 5uM) for a protein that is involved in a specific pathway. Now I am doing cell-based assays to see the In-vivo effect. I, Incubate the cpds with the...
01 February 2022 8,826 3 View
I am trying to see the effect of drugs on my cellular target. Before that, I have to make sure that they are not toxic to the cells. As such, I am performing cell viability assay using alamarblue...
12 October 2021 603 3 View
Hello everyone, In the paper, Petsch et al., Nature 2012 mentions these points, •mRNA is naturally recognized by innate pattern-recognition receptors (Effective immune response. Reducing the...
21 September 2020 1,860 4 View
Greetings everyone, I am looking for an assay suitable to detect atleast, 500nM to 1mM ATP hydrolysis. My Kinase has a very weak activity. The signal should be good enough (also must be...
14 August 2020 3,369 1 View
Hello everyone, I am screening for Inhibitors. Km of my enzyme is 20uM. but at 20uM substrate conc I am not getting a good signal. The graph curves very quick and also the signal to noise ratio...
29 June 2020 7,662 0 View
The context is, that the inhibitor binds to the viral protease inside the host cell (virus has entered the host cell) and prevents viral replication. In the catalytic site, there is one covalent...
05 May 2020 6,441 4 View
I want to see the cellular effect of my compounds found via high throughput screening. I am using MEF for my cell-based assays. The compounds I have dissolved in DMSO (synthesis grade)...
01 January 1970 9,447 4 View
