Mohd Saif

8 Questions 9 Answers 0 Followers

Questions related from Mohd Saif

Cell based assay to see the effect of Inhibitors?

via HTS I found Inhibitors (IC50 between 1 to 5uM) for a protein that is involved in a specific pathway. Now I am doing cell-based assays to see the In-vivo effect. I, Incubate the cpds with the...

01 February 2022 8,884 3 View

Incubation time of MEF´s (mouse embryonic fibroblast) with alamarblue in cell viability assay?

I am trying to see the effect of drugs on my cellular target. Before that, I have to make sure that they are not toxic to the cells. As such, I am performing cell viability assay using alamarblue...

12 October 2021 652 3 View

mRNA vaccine advantage contradicting the current covid mRNA vaccine being developed?

Hello everyone, In the paper, Petsch et al., Nature 2012 mentions these points, •mRNA is naturally recognized by innate pattern-recognition receptors (Effective immune response. Reducing the...

21 September 2020 1,982 4 View

ADP-Glo assay vs Kinase Glo assay? Or an assay for weak kinase activity?

Greetings everyone, I am looking for an assay suitable to detect atleast, 500nM to 1mM ATP hydrolysis. My Kinase has a very weak activity. The signal should be good enough (also must be...

14 August 2020 3,413 1 View

Inhibitor screening, how important is that your substrate conc. be lower or equal to Km?

Hello everyone, I am screening for Inhibitors. Km of my enzyme is 20uM. but at 20uM substrate conc I am not getting a good signal. The graph curves very quick and also the signal to noise ratio...

29 June 2020 7,713 0 View

Inhibitor binding covalently in the catalytic site. How will it be released?

The context is, that the inhibitor binds to the viral protease inside the host cell (virus has entered the host cell) and prevents viral replication. In the catalytic site, there is one covalent...

05 May 2020 6,499 4 View

Brain vasculature. Are there blood vessels inside the brain? after the Blood brain barrier (BBB)?

Hello everyone, I was trying to understand the nutrient uptake in the brain. how does the blood flow regulate it? If it is only limited to BBB, how do neurons which are centrally located get the...

14 November 2019 9,380 3 View

Inhibitors in DMSO (not for cell culture) can be used in cellular assays?

I want to see the cellular effect of my compounds found via high throughput screening. I am using MEF for my cell-based assays. The compounds I have dissolved in DMSO (synthesis grade)...

01 January 1970 9,501 4 View