2nd PCR please help?

I am trying to clone mouse Mrp4 (started from cDNA), after designing four primers (4-1R,F,,4-2 F,R,, 4-3 F,R,, 4-4 F,R) with overlap more than 20 bases, I got four segments around 1000bp(expected...

09 October 2019 2,077 3 View

Skin staining protocol ?

hello everyone I am really in need of an H&E staining protocol of mouse skin, looking forward for your generous help!

09 October 2019 1,327 3 View

Gene cloning of Mrp4?

Hello everyone I would like to ask your help in understanding some point, I am trying to clone a very long gene around 4000bp , I used the method of HiFi assembly Kit which require designing...

08 September 2019 7,156 3 View

Good pharmacokinetics book?

Hello every one I am in need of your precious suggestions on good pharmacokinetics book. I am currently doing research on drug transporters and drug response and their effect on...

05 June 2018 4,133 1 View

Hello every one ?

I am doing a dose response studies using wild type and mutant mice missing certain transporter, to test the pharmacokinetics of anti cancer drug. But I am in need of a statistical test to test...

04 May 2018 3,667 1 View

Non linear pharmacokinetics ?

hello everyone I have performed a dose acceleration experiment But I got linear plasma profile However There was a significant decrease in the liver and kidney concentration of the drug when...

03 April 2018 333 3 View

RT PCR problem can any one help me why I'm getting such curve ?

I used RNAase mini kit to isolate my RNA

01 February 2018 5,426 8 View

Hello every one I need help in doing a skin toxicity studies ?

I'm trying to test the toxic effects of anticancer drug on the skin .this drug will be given orally as once daily dose for 5 days . which bio marker should I measure also do you have any...

11 December 2017 8,618 4 View

Hello every one Im a beginner inlcmsms.I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded ?

I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded verapamil 50nM and made a calibration standreds from 1nM to 3000nM but the problem is in...

02 March 2017 7,488 3 View

Can anyone help me please , I want to prepare a drug solution of 0.06 M drug , and all i know is the solubility of this drug is 97 mg/ml in DMSO ?

01 February 2017 3,256 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View