Inflammation in mice feet ?

08 August 2019 1 3K Report

Hello everyone

I am trying to asses the inflammation in mice feet but I am a bit confused about the best way to measure the inflammation if I can use any device .

please help me with your valuable suggestions

Prof. Gheita

Dear Ella Hasan

Nice Q

Check these link

https://www.researchgate.net/post/How_to_measure_mice_footpad_swelling_inflammation

Article Mouse paw edema. A new model for inflammation?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Ella Hasan's questions See All

2nd PCR please help?

I am trying to clone mouse Mrp4 (started from cDNA), after designing four primers (4-1R,F,,4-2 F,R,, 4-3 F,R,, 4-4 F,R) with overlap more than 20 bases, I got four segments around 1000bp(expected...

09 October 2019 2,154 3 View

Skin staining protocol ?

hello everyone I am really in need of an H&E staining protocol of mouse skin, looking forward for your generous help!

09 October 2019 1,446 3 View

Gene cloning of Mrp4?

Hello everyone I would like to ask your help in understanding some point, I am trying to clone a very long gene around 4000bp , I used the method of HiFi assembly Kit which require designing...

08 September 2019 7,245 3 View

Good pharmacokinetics book?

Hello every one I am in need of your precious suggestions on good pharmacokinetics book. I am currently doing research on drug transporters and drug response and their effect on...

05 June 2018 4,218 1 View

Hello every one ?

I am doing a dose response studies using wild type and mutant mice missing certain transporter, to test the pharmacokinetics of anti cancer drug. But I am in need of a statistical test to test...

04 May 2018 3,745 1 View

Non linear pharmacokinetics ?

hello everyone I have performed a dose acceleration experiment But I got linear plasma profile However There was a significant decrease in the liver and kidney concentration of the drug when...

03 April 2018 418 3 View

RT PCR problem can any one help me why I'm getting such curve ?

I used RNAase mini kit to isolate my RNA

01 February 2018 5,513 8 View

Hello every one I need help in doing a skin toxicity studies ?

I'm trying to test the toxic effects of anticancer drug on the skin .this drug will be given orally as once daily dose for 5 days . which bio marker should I measure also do you have any...

11 December 2017 8,697 4 View

Hello every one Im a beginner inlcmsms.I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded ?

I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded verapamil 50nM and made a calibration standreds from 1nM to 3000nM but the problem is in...

02 March 2017 7,613 3 View

Can anyone help me please , I want to prepare a drug solution of 0.06 M drug , and all i know is the solubility of this drug is 97 mg/ml in DMSO ?

the drug will be used to do in vivo experiment

01 February 2017 3,345 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can i do to find the right topic for my thesis?

i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.

02 August 2024 1,323 8 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View