2nd PCR please help?

I am trying to clone mouse Mrp4 (started from cDNA), after designing four primers (4-1R,F,,4-2 F,R,, 4-3 F,R,, 4-4 F,R) with overlap more than 20 bases, I got four segments around 1000bp(expected...

09 October 2019 2,077 3 View

Skin staining protocol ?

hello everyone I am really in need of an H&E staining protocol of mouse skin, looking forward for your generous help!

09 October 2019 1,327 3 View

Gene cloning of Mrp4?

Hello everyone I would like to ask your help in understanding some point, I am trying to clone a very long gene around 4000bp , I used the method of HiFi assembly Kit which require designing...

08 September 2019 7,156 3 View

Inflammation in mice feet ?

Hello everyone I am trying to asses the inflammation in mice feet but I am a bit confused about the best way to measure the inflammation if I can use any device . please help me with your...

07 August 2019 2,704 1 View

Good pharmacokinetics book?

Hello every one I am in need of your precious suggestions on good pharmacokinetics book. I am currently doing research on drug transporters and drug response and their effect on...

05 June 2018 4,133 1 View

Hello every one ?

I am doing a dose response studies using wild type and mutant mice missing certain transporter, to test the pharmacokinetics of anti cancer drug. But I am in need of a statistical test to test...

04 May 2018 3,667 1 View

Non linear pharmacokinetics ?

hello everyone I have performed a dose acceleration experiment But I got linear plasma profile However There was a significant decrease in the liver and kidney concentration of the drug when...

03 April 2018 333 3 View

RT PCR problem can any one help me why I'm getting such curve ?

I used RNAase mini kit to isolate my RNA

01 February 2018 5,426 8 View

Hello every one Im a beginner inlcmsms.I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded ?

I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded verapamil 50nM and made a calibration standreds from 1nM to 3000nM but the problem is in...

02 March 2017 7,488 3 View

Can anyone help me please , I want to prepare a drug solution of 0.06 M drug , and all i know is the solubility of this drug is 97 mg/ml in DMSO ?

01 February 2017 3,256 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View