2nd PCR please help?

I am trying to clone mouse Mrp4 (started from cDNA), after designing four primers (4-1R,F,,4-2 F,R,, 4-3 F,R,, 4-4 F,R) with overlap more than 20 bases, I got four segments around 1000bp(expected...

09 October 2019 2,077 3 View

Skin staining protocol ?

hello everyone I am really in need of an H&E staining protocol of mouse skin, looking forward for your generous help!

09 October 2019 1,327 3 View

Gene cloning of Mrp4?

Hello everyone I would like to ask your help in understanding some point, I am trying to clone a very long gene around 4000bp , I used the method of HiFi assembly Kit which require designing...

08 September 2019 7,156 3 View

Inflammation in mice feet ?

Hello everyone I am trying to asses the inflammation in mice feet but I am a bit confused about the best way to measure the inflammation if I can use any device . please help me with your...

07 August 2019 2,704 1 View

Good pharmacokinetics book?

Hello every one I am in need of your precious suggestions on good pharmacokinetics book. I am currently doing research on drug transporters and drug response and their effect on...

05 June 2018 4,133 1 View

Non linear pharmacokinetics ?

hello everyone I have performed a dose acceleration experiment But I got linear plasma profile However There was a significant decrease in the liver and kidney concentration of the drug when...

03 April 2018 333 3 View

RT PCR problem can any one help me why I'm getting such curve ?

I used RNAase mini kit to isolate my RNA

01 February 2018 5,426 8 View

Hello every one I need help in doing a skin toxicity studies ?

I'm trying to test the toxic effects of anticancer drug on the skin .this drug will be given orally as once daily dose for 5 days . which bio marker should I measure also do you have any...

11 December 2017 8,618 4 View

Hello every one Im a beginner inlcmsms.I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded ?

I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded verapamil 50nM and made a calibration standreds from 1nM to 3000nM but the problem is in...

02 March 2017 7,488 3 View

Can anyone help me please , I want to prepare a drug solution of 0.06 M drug , and all i know is the solubility of this drug is 97 mg/ml in DMSO ?

01 February 2017 3,256 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View