2nd PCR please help?

I am trying to clone mouse Mrp4 (started from cDNA), after designing four primers (4-1R,F,,4-2 F,R,, 4-3 F,R,, 4-4 F,R) with overlap more than 20 bases, I got four segments around 1000bp(expected...

09 October 2019 2,077 3 View

Gene cloning of Mrp4?

Hello everyone I would like to ask your help in understanding some point, I am trying to clone a very long gene around 4000bp , I used the method of HiFi assembly Kit which require designing...

08 September 2019 7,156 3 View

Inflammation in mice feet ?

Hello everyone I am trying to asses the inflammation in mice feet but I am a bit confused about the best way to measure the inflammation if I can use any device . please help me with your...

07 August 2019 2,704 1 View

Good pharmacokinetics book?

Hello every one I am in need of your precious suggestions on good pharmacokinetics book. I am currently doing research on drug transporters and drug response and their effect on...

05 June 2018 4,133 1 View

Hello every one ?

I am doing a dose response studies using wild type and mutant mice missing certain transporter, to test the pharmacokinetics of anti cancer drug. But I am in need of a statistical test to test...

04 May 2018 3,667 1 View

Non linear pharmacokinetics ?

hello everyone I have performed a dose acceleration experiment But I got linear plasma profile However There was a significant decrease in the liver and kidney concentration of the drug when...

03 April 2018 333 3 View

RT PCR problem can any one help me why I'm getting such curve ?

I used RNAase mini kit to isolate my RNA

01 February 2018 5,426 8 View

Hello every one I need help in doing a skin toxicity studies ?

I'm trying to test the toxic effects of anticancer drug on the skin .this drug will be given orally as once daily dose for 5 days . which bio marker should I measure also do you have any...

11 December 2017 8,618 4 View

Hello every one Im a beginner inlcmsms.I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded ?

I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded verapamil 50nM and made a calibration standreds from 1nM to 3000nM but the problem is in...

02 March 2017 7,488 3 View

Can anyone help me please , I want to prepare a drug solution of 0.06 M drug , and all i know is the solubility of this drug is 97 mg/ml in DMSO ?

01 February 2017 3,256 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View