Hi,
So I am trying to use a Hitachi F7000 fluorometer to measure the photoluminescence of thin films on glass. When I measure only the glass, I get strongly varying spectra, depending on the excitation wavelength. Attached you see spectra on the same sample using either 350 nm or 400 nm excitation, the results being strikingly different. Any help is greatly appreciated.
Conventional glass microscope slides of complex composition (https://brainresearchlab.com/wp-content/uploads/2017/02/Slide-Composition-Sheet.pdf), and therefore they have very diverse luminescence from various ions and their associated defects. The most reliable way to avoid luminescence is to use fused quartz microscope slides (https://www.uqgoptics.com/catalogue/laboratory/microscope-slides/fused-quartz-microscope-slides/). Also I know that when annealing optical filter from glass near the softening point (400-450 °C) you can reduce the luminescence, maybe this can help.
I'm curious whether a short wavelength source damaged your sample since it provides higher energy. Maybe measurement using longer wavelengths is better in this case? Also, you can try to reduce the power of the laser source and/or reduce the exposure time.
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