In which media should I subculture pseudomonas syringae from pure culture?

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How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

Is VSM measurment of NdFeB alloy powder sample require any spesific sample preparation ?

Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...

09 July 2024 1,969 0 View

How to see whether my protein is aggregated using western blotting?

I am working on one protein which gets aggregated (similar to alpha-synuclein and tau) in certain neurodegenerative diseases. I am trying to confirm the aggregation using Western blotting....

30 June 2024 5,060 3 View

How can i calculate the band alignmet of Sc2CO2/BlueP heterostructure from DFT?

I know the work function and band gap of each monolayer and heterostructure. I calculated mulliken electronegativity(X) of Sc2CO2 and Bluep. From that i found Ev = X- 4.5+Eg Ec = Ev-Eg, where Eg...

28 June 2024 9,360 0 View

Are all indexing bodies for the journal's evaluation similar?

A Guide to Journal Indexing Academic journals undergo evaluation by various indexing bodies to ensure ethical practices and high publishing standards. Three prominent indexes include: · PubMed...

27 June 2024 4,415 0 View

How to get Consistency Ratio when scale of relative importance is used in AHP?

In AHP (Analytical Hierarchical Process) using 9 criteria, when I use a a scale of relative importance (1-9), I am getting a higher lambda-max and hence an inconsistent Consistency Ratio (>.10)...

16 June 2024 1,228 1 View

What are applications of Class 1, 2, 3 and 4 steel s/c? Can Class 4 s/c considered as flexural members? Why are Class 4 sections not adopted as beams?

Where are Class 1, 2, 3 and 4 sections generally adopted by steel construction industy? Why are class 4 sections not used as beams? As what structural members are Class 4 members used?

10 June 2024 2,453 1 View

Can we use any inferential statistics on a data set measured using non-validated questionnaires?

I have heard from my senior scholars that we can't use a non-validated scale to measure variables in a correlational study. Is it true? Can anyone help me with finding a reference for the same.

05 June 2024 7,851 4 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How to interpret results of AST for Pseudomonas aeruginosa?

While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...

25 July 2024 9,229 1 View

Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View

Why pet plasmids can't replicate in pseudomonas?

Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and...

25 June 2024 7,083 1 View

What plasmid is good for cloning in pseudomonas?

Hi, does anyone khonw which plasmid can we use in cloning in pseudomonas? I have to overexpress two genes in pseudomonas and I don't know which plasmid can replicate and express in pseudomonas....

24 June 2024 9,240 3 View

Baseic questions in pseudomonas cloning?

Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...

24 June 2024 5,307 2 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

How are cancerous cell lines generated in 1900s sold today have the same genetic profile?

From the definition of cell line, they are indefinitely propagated/subcultured. However, in practice beyond a certain passage number, they are said to loose characteristic gene profile, for e.g....

10 June 2024 6,890 1 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View