In western blue, what average dilution for these antibodies : cyclin a and d, caspase 3,9, PCNA?

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Why the clear zone indicating amylase activity on agar plates dissappears after some hours?

I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...

13 May 2024 6,529 2 View

My recombinant enzyme activity is acting weird, does anyone have an explanation, or even know how to solve this problem?

I'm working with recombinant protein, it's a protease enzyme that we use E. coli to synthesize, and usually, it's stable for up to six months in the -80 freezer. Recently, the protein activity has...

17 April 2024 4,262 3 View

Sub-cloning not working?

I am a graduate student doing sub cloning of two similar genes into pBC SK(+); one of the gene was inserted but few colonies appeared on the agar after transformation, the other is not inserting...

22 February 2024 7,237 5 View

How can audiologists enhance patient-centered care in their practice?

In light of recent research findings and advancements in communication strategies for individuals with hearing loss, how can audiologists enhance patient-centered care in their practice? Are there...

17 December 2023 2,894 1 View

Why does performance point still in elastic range ?

I have a four-story steel structure When conducting a pushover analysis and examining the results, the structure's ductility ratio at the performance point did not exceed 1, and the equivalent...

08 November 2023 934 4 View

Cadmium sulfide nanoparticles from banana extract , what is the problem?

I'm preparing Cadmium sulfide nanoparticles using banana extract as a capping agent, after drying it, it looks like thin sheets, cannot be grinded as powder (The last time, it does not look like...

12 June 2023 3,604 1 View

Safe cities for women with disabilities ?

Safe cities for women with disabilities

13 May 2023 8,560 3 View

Can I use QIAamp DNA Stool Mini Kit for DNA extraction from a bacterial colony? if anyone tried this and what are the necessary protocol adjustments?

#DNA_extraction #QIAamp #Qiagen #Colony_DNA

04 May 2023 4,548 2 View

How to solve the problem if the sensor is not capable of transmitting data to base station in WSN. Is there any paper talking about this issue?

25 January 2023 5,399 4 View

Why enzymes couldnot migrate from wells to stacking gel in electrophoresis? Is there is problem in its precipitation?

i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...

10 January 2023 2,353 5 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View