TFBS analysis, these days, has encountered two interesting approaches in predicting the binding sites, in one hand we have Position Weight Matrix which is a contextual-based protocol and in the other we have SITECON which is based on conformational/physicochemical properties of the binding sites. We acknowledge this point that the ability to work on predicted TFBSs based on conformational properties is very attracting but the question is that there is any necessity to use Sitecon on bacterial genome?