In situ hybridization?

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What is the LD50 of Melissa officinalis?

Hello, What is the lethal dose (LD50) of the essentiel oïl of Melissa officinalis in Wister Rats? I want information from articles Thanks for All

10 November 2018 7,372 0 View

How to publish a Book?

How to publish a book in a publishing house such as Dunod, Masson or willy ...?

08 September 2018 7,038 0 View

Hello. I have a problem. It's been a while since I can no longer follow anyone??

Hello. I have a problem. It's been a while since I can no longer follow anyone. Whenever you press the "follow" icon, a red banner appears where this writing "You've reached your limit of...

10 November 2017 6,129 6 View

Centrifugation between the number of Revolutions (rmp) and time??

Centrifugation between the number of laps and time?? The number of sessions per second can be compensated. Or the speed of the rotation. The duration of the centrifuge. I mean, could we double the...

09 October 2017 7,962 4 View

What is the difference between the cytosol and the s9 fraction?

-To evaluate a biochemical or enzymatical parameters in tissue take the cell cytosol of these tissue. But according to research: after differential centrifugation there are a lot of cytosoliques...

04 May 2017 1,079 2 View

What is the optimal concentration of formaldehyde for the realization of good histological study of the full brain (brain completely) of rats ?

The objectif of this histological study is the comparison between the different regions of brain in rats. Questions: - What is the optimal concentration of formaldehyde for the realization of good...

03 April 2017 4,844 8 View

Question of the Day – Tuesday March 6, 201ا8?

Which drugs and chemicals have been reported to cause false positive urine immunoassay screen results for PCP?

01 January 1970 628 0 View

What is "alternative oxidase"?. Is that there in humans or only in plants?

Alternative oxidase (AOX): The alternative oxidase (AOX) is an enzyme that forms part of the electron transport chain in mitochondria of different organisms. Proteins homologous to the...

01 January 1970 5,328 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View