What is the LD50 of Melissa officinalis?

10 November 2018 7,213 0 View

How to publish a Book?

How to publish a book in a publishing house such as Dunod, Masson or willy ...?

08 September 2018 6,913 0 View

Hello. I have a problem. It's been a while since I can no longer follow anyone??

Hello. I have a problem. It's been a while since I can no longer follow anyone. Whenever you press the "follow" icon, a red banner appears where this writing "You've reached your limit of...

10 November 2017 5,992 6 View

Centrifugation between the number of Revolutions (rmp) and time??

Centrifugation between the number of laps and time?? The number of sessions per second can be compensated. Or the speed of the rotation. The duration of the centrifuge. I mean, could we double the...

09 October 2017 7,852 4 View

In situ hybridization?

I'm looking for a brief explanation of this method. Defined. its role. Its importance. Its mechanism. How to work it out. Its principle. The tools used. Thank you for your attention.

09 October 2017 3,391 4 View

What is the difference between the cytosol and the s9 fraction?

-To evaluate a biochemical or enzymatical parameters in tissue take the cell cytosol of these tissue. But according to research: after differential centrifugation there are a lot of cytosoliques...

04 May 2017 972 2 View

What is the optimal concentration of formaldehyde for the realization of good histological study of the full brain (brain completely) of rats ?

The objectif of this histological study is the comparison between the different regions of brain in rats. Questions: - What is the optimal concentration of formaldehyde for the realization of good...

03 April 2017 4,740 8 View

What is "alternative oxidase"?. Is that there in humans or only in plants?

Alternative oxidase (AOX): The alternative oxidase (AOX) is an enzyme that forms part of the electron transport chain in mitochondria of different organisms. Proteins homologous to the...

01 January 1970 5,233 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View