In simple terms: to what extent do you believe classicial inversion frameworks provide more "general" estimates than learned frameworks?

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How should we use processes of experimentation to improve our urban spaces?

Myself, Dr Michael Crilly and Dr Georgiana Varna are currently engaged in developing and deploying a series of practical, urban design, experiments in the North East of England. We are doing this...

20 June 2024 7,559 10 View

What (preferably UK-based) Psychology journals exist that focus on publication of undergraduate and postgraduate student work?

I'm looking for an appropriate journal in which to publish the dissertation research of one of my undergraduate Psychology students. This student died by suicide recently, so I wanted to get...

28 April 2024 7,486 2 View

How to best design shRNA?

this is the first time I design shRNA and I have looked online but couldn't find the answer to the following questions: - should I add an overhang? what overhang do you suggest? dtdt? - I am...

23 October 2023 3,412 0 View

How to know if CRISPR did heterozygous or homozygous knock-out?

Hello everyone I have been doing CRISPR to knockout a gene from my HEK-293 cell line. I have transfect with SgRNA-cas9 and now my cells have been growing for a week. I am planning on doing DNA...

08 August 2023 4,401 12 View

Does anyone have the data from the Google Scholar Metrics 2022 for the journals (name, h5-index, h5-median) in Spanish/English?

I want to make a comparison of the rankings of journals in Spanish and English on Google Scholar Metrics since 2020, but the URL...

31 July 2023 6,686 0 View

Cloning sgRNA into Px458, no successful cloning so far?

I have been tring to clone a 25 bp(5`-CACCGXXXX....-3` and 5`-AAACXXXX...C-3`) sgRNA into Px458. I have been using this method :...

27 April 2023 3,621 3 View

Can I visualize a 78 bp DNA fragment on agarose gel?

Hello I am trying to cut a segment from a plasmid that is around 80 and I wonder if I could see it on the gel taking in consideration that the smallest size on the ladder is 100 bp. however, I...

25 April 2023 9,357 3 View

Is it OK to leave LB media with antibiotics in it on the self in room temprature?

I usually pre-prepare media with antibiotics and put in on the shelf for quick access (like doing minipreps). I wonder if that would effect the antibiotics in the media and if it would cause any...

11 April 2023 6,374 3 View

Can I use the same sgRNA or siRNA that was used previously in a published study?

I want to do CRISPR and ShRNA for few genes and I came a cross few studies that knocked-out those genes and they shared in the method sections the sequences that worked. usually for CRISPR and...

27 March 2023 6,112 0 View

Any advices about generating CRISPR-KO cell line?

I am trying to knock out a gene in a cell line using CRISPR and I have been reading alot about that. I want to use 2 gRNA (crRNA and tracer RNA and not sgRNA) to do a large deletion in a gene. can...

16 January 2023 5,274 1 View

Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View