I am working on fungus which is asexually reproducing. I have three populations with 14, 28 and 38 isolates (samples). After analyzing the ISSR data set I found low values of pairwise Fst. Now my questions is, what are the factors which affect the Fst values other than gene flow?
One possible reason for low values of Fst may be due to similar allele frequencies within each population. Fst values are directly related to variation in allele frequencies among populations.
Hi,
Is your fungus haploid or diploid? ISSR are usually dominant markers, so they are not well suited to detect heterozygotes, which is one basis of Fst indexes.
Best regards
Didier
Fst for a diploid locus is good, but for a haploid locus it is not at all god, because in haploids heterozygosity is meaningless, thus Fst. For haploids its better to go for haplotye diversity instead of heterozygosisty.
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