Python - TRNSYS link not working with Type169?

I used Type169 for data exchange between Python and TRNSYS. Unfortunately, facing the following error. No proper documentation is available for this part. TRNSYS Message 105: A TYPE was called in...

14 February 2021 5,577 1 View

What is the role of ether solvent (such as THF) in Sonogashira coupling?

Can Amine (such as Et3N) be used as solvent as well as base for the Sonogashira Coupling? How to decide the amount of solvent to be used in any reaction? Does the amount of solvent affect the...

14 February 2021 4,018 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

How can i calculate diffusion barrier of Li ion on graphene nanoribon using SIESTA?

I'm a new siesta learner, I'm working on lithium-ion batteries, I have calculated adsorption energy but i am not able to calculate diffusion barrier of Li ion. kindly help me how to calculate...

14 January 2021 2,688 1 View

Suggest some good journals having Impact factor more than 3 which is not an open access one for alternative and complimentary medicine?

Suggest some good journals having Impact factor more than 3 which is not an open access one for alternative and complimentary medicine?

11 January 2021 7,782 1 View

Can I use hcl to adjust ph rather than glacial acetic acid?

In alkaline lysis solution3 acetic acid should be used to adjust pH according to protocol. Can I use hcl instead of glacial acetic acid? Will there be any change in composition of solution?

08 January 2021 7,764 3 View

What is the effect of polarization of unit elements in an IRS, if its built for mmWave application?

I usually don't see path loss models for IRS consider polarization. But specific to mmWave application, the polarization of the antenna has some effect. Is there any research that has explored this?

08 January 2021 3,054 1 View

What is the best method for plasmid dna extraction??

I want to extract plasmid dna from bacterial culture guide me what will be the most appropriate method.

05 January 2021 1,118 14 View

What could be the role of researchers to reduce violence in school?

I want to promote peace and non-violence in school and collaborate for such initiative in future.

30 December 2020 973 3 View

How to reduce liquation cracking in super austenitic stainless steels ?

Super austenitic stainless steel castings showed liquation crack after liquid penetrant testing post heat treatment. How to reduce this in thick walled castings.

26 December 2020 7,959 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View