1- What macrophage is best to work (peritoneum or differentiate from bone marrow or kupffer cells)?
2- Have some problem using thioglycollate to amount macrophages?
Are you interested in evaluating transcript expression or protein? If I am performing in vitro pilot experiments, I tend to use peritoneal macrophages without using thioglycollate since this can elicit an activated phenotype in the macrophages and consequently may cause unwanted alteration of genes.
Hi Anne,
First of all, there is no gene whose official symbol is Pcg-1. I am sure that there is a gene whose informal name is Pcg-1, but to me it is not clear at all which gene you are working with. http://www.ncbi.nlm.nih.gov/gene is a good source to find the official name for a gene.
You have at least two startegies here: literature search and online databases. For both of them, you'd better to use the official name. http://www.immgen.org/databrowser/index.html is a good online source to see what is the gene expression value of any gene in murine immune cells. Using a dropdown list, you can limit your search to Mfs, moncytes etc, or you can check the expression in a huge array in (unrelated) immune cells.
Independently of your assay, be aware that BMDMacs and peritoneal Macs do not respond similarly to the same stimulus. Many times you do not get the same results. Example: expression of SOCS 3 is on after mature BMDMacs, but SOCS 3 is not expressed on perotineal macrophages.
- Badges
- Science method