Just curious if measuring glycemic and cholesterol parameters in mice after fasting them for 6 hours gives more accurate read -outs than fed state.
Fasting gives you more results when it comes to glycemic levels, but regarding cholesterol parameters, fasting is not necessary. Do you use weight gain as surrogate of postprandial hyperinsulinemia? I do.
Appropriate answer in the abstract in JACC-
A Test in Context: Lipid Profile, Fasting Versus Nonfasting
Børge G. Nordestgaard
J Am Coll Cardiol. 2017 Sep, 70 (13) 1637–1646
Abstract
Fasting for >8 h, as previously required for lipid profiles, normally only occurs a few hours before breakfast. By contrast, the nonfasting state predominates most of a 24-h cycle and better captures atherogenic lipoprotein levels. Plasma contains atherogenic lipoproteins of hepatic origin in the fasting state and additionally those of intestinal origin in the nonfasting state. Maximal mean changes for random, nonfasting versus fasting levels are +26 mg/dl for triglycerides, −8 mg/dl for total cholesterol, −8 mg/dl for low-density lipoprotein cholesterol, +8 mg/dl for remnant cholesterol, and −8 mg/dl for non–high-density lipoprotein cholesterol; lipoprotein(a), apolipoprotein B, and high-density lipoprotein cholesterol are largely unaffected. For patients, laboratories, and clinicians alike, nonfasting lipid profiles represent a simplification without negative implications for prognostic, diagnostic, and therapeutic options for cardiovascular disease prevention. Several societies’ guidelines and statements in Denmark, the United Kingdom, Europe, Canada, Brazil, and the United States endorse nonfasting lipid profiles.
I agree with Silvia Clapauch. Epidemiologic studies of cholesterolemia can be without fasting. If you make lipid profile is different. The tryglicerides levels can be altered by non fasting
Fasting gives more accurate results for both glycemic and cholesterol examination.
But if you want to check glucose tolerance then perform fasting test first then go for random after glucose intake
- Badges
- Science topic
- Similar topics
- Mathematics
- Statistics
- Sampling Design
- Sample Size