I have a list of identifiers from my IP samples (protein-tag and only tag expressing). I am trying to filter data of common identifiers in both samples. What parameter from the data that I should consider in this case. Thanks
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints when entering K alpha value. Because of that I am not able to find the peaks...
11 June 2024 4,018 2 View
I am new to computational calculations and it is very difficult to select a pc or laptop for quantum chemical calculation. It would be great if anyone just give me a list contains specified type...
09 June 2024 4,988 2 View
I have a xrd data of a sample to be analyzed for calculating its residual stress. Any suggestions might be helpful
19 March 2024 942 3 View
Greetings of the day, Dear Friends, I hope you are doing well. Kindly discuss the general pipeline/methodology/steps to do Next-generation sequencing (NGS). Can Anyone tell me where i can learn...
07 March 2024 555 4 View
Hlo I am trying to get kinetics of methanol to dimethyl ether kinetics on hzsm-5 si/al = 40 catalysts at 160 deg c I was unable to reach zeroth order kinetic regime. I doubt that the reaction will...
05 March 2024 7,671 1 View
Hello everyone, I am trying to run Rietveld refinement on my sample which has two phases. The primary phase is quite dominant with most of the peaks, but there is only one peak of the secondary...
04 March 2024 9,832 2 View
Research papers on SCs mention capacitance in terms of both specific and areal capacitance. How does one decide which one to use while reporting? Thanks in advance
28 January 2024 5,721 1 View
I want to perform GCD for symmetric as well as asymmetric supercapacitor devices. In that regard, can anyone suggest specific current (consideration of the mass of one or the sum of two electrode...
28 January 2024 3,677 1 View
I have a list of identifiers/hits processed based on log fold changes. I tried using pantherDB to generate GO terms and cytoscape for network construction. I need a clear picture on how to...
02 January 2024 8,770 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View