In Mass spectrometry data, how do I compare my identifiers with that from the background?

Vignesh Venkadasubramanian @Vignesh-Venkadasubramanian

23 March 2023 0 4K Report

I have a list of identifiers from my IP samples (protein-tag and only tag expressing). I am trying to filter data of common identifiers in both samples. What parameter from the data that I should consider in this case. Thanks

Badges
Science method

More Vignesh Venkadasubramanian's questions See All

How can I analyse the data from case study in qualitative research ?

When I use purposive sampling method especially homogenous sampling to select the participants and then followed by my selection process the data can be collected by using case study for my...

13 November 2020 6,411 7 View

What are the main tools used to analyze the data which is collected from the qualitative social work research ?

i have used case study and non participant observation as a research methods in the qualitative research , hence i need some tools to analyze the collected data....I've belongs to the social work...

25 October 2020 438 8 View

Anyone knows about .odb_f file generated by ABAQUS?

Hello all, I'm running a Bone Remodeling simulation in ABAQUS in which I just use basic ABAQUS Static simulations in every loop with an amplitude table to model the loading behavior & after...

19 October 2020 4,417 2 View

How to apply distributed load in a 2D model with plane stress/plane strain thickness in ABAQUS?

Hello all, I'm currently working on setting-up a 2D bone remodeling problem of a femur from literature. I take a 2D femur geometry and give it an out-of plane plane stress/plane thickness in...

06 October 2020 2,623 5 View

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods?

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods? if possible such as 1. Calorimetric or Spectroscopic methods and much more

26 August 2020 349 10 View

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

02 March 2020 1,011 2 View

What are all the possible reasons for Spontaneous symmetry breaking in a double quantum well system?

Dear frnds, From my point of view SSB is happening due to following reasons in double quantum wells. 1. Due to variational technique 2. symmetry in envelop function and...

19 October 2019 6,248 2 View

How to account for Depth of cut in 2D orthogonal cutting simulation in Abaqus ?

I am making a Dynamic Temp-Disp Explicit simulation of 2D orthogonal metal cutting operation in abaqus. What is the depth of cut of the above simulation. The unit I have assumed for the simulation...

22 April 2019 3,779 8 View

Why the cutting force plot differs drastically with change in speed ?

I have made a Dynamic explicit analysis of 2D metal cutting operation with Abaqus. I have plotted the reaction force in X axis of the node at the tool tip. I had run a similar simulation with...

12 April 2019 7,398 7 View

What is the significance of size dependent dielectric screening in optimizing excitonic properties?

Mott-Wannier excitons are formed in a semiconductor material as a result of light-matter interactions. However, their delocalizing properties inside the crystal induce screening effects in their...

10 February 2019 6,737 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View