What should be sample size of traning dataset for applying data mining techniques?

21 December 2020 8,119 3 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Is it possible to calculate Absorption co-efficient or Absorbance of a dye theoretically?

I am working on a Luminescent Solar Concentrators (LSC) using dyes such as Coumarin 1, Coumarin 153, and Perylene Green Ph-probe 720-5.5. I need to calculate the Mean-free-path (MFP) for the...

18 August 2020 9,804 3 View

Is ACE inhibition linked to COVID-19?

There is increasing chatter that proteins in the ACE2 receptor binding domain are related to COVID-19 entering humans cells and subsequently infecting the host. Is there any evidence of ACE...

19 March 2020 6,635 8 View

In Phospharic Acid plant , Calendria is being used is generally of Graphite tube- shell & tube heat exchanger. Anyone using experience SS316 tubes?

Presently Majority of PA plants are using Graphite tubes in their system oh concentration for heat-exchange. Reliability is little less. We want to know that is ANY PA producing plants is having...

13 March 2020 4,877 3 View

How to model blind bolts in ABAQUS?

I am trying to simulate a beam-column connection using blind bolts. but I am not getting an idea to model blind bolts in Abaqus. please give me some idea of modelling. thanks in advance

05 February 2020 8,916 3 View

What are the possible ways to increase the internodal distance of in vitro cultures?

I am working on Papaya micropropagation where I am getting plenty of shoots but they are not increasing in height. I have tried diffuse light, GA3 but did not see improvement. Please suggest a...

03 June 2019 812 23 View

Reduction in fasting Insulin levels and associated CVD/T2D risk?

Does anyone have papers relating fasting insulin levels and associated CVD risks?

03 November 2018 4,281 3 View

What is the relationship between Stroke Volume and Diastolic BP?

Does stroke volume and diastolic BP have a positive linear correlation? Given greater pre-load and thus end-diastolic volume is it expected that diastolic BP would be reduced?

14 December 2017 9,316 4 View

Is RM-ANOVA robust enough with a non-normal dataset?

Does anyone have papers saying that repeated-measures ANOVA has sufficient robustness when analysing a non-normal dataset?

30 November 2017 7,862 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View