Darshini Desai

6 Questions 23 Answers 0 Followers

Questions related from Darshini Desai

In house Immunostaining protocol for cardiac organoids?

Hi researchers! Does anyone have a in-house immunostaining protocol to stain for organoids? I am guessing my antibody is not able to permeate well. If anyone has any in house protocol that works...

02 November 2021 5,797 7 View

Measuring contractility in hiPSC-CMs?

Hi Guys, Has anyone tried measuring contractility in hiPSC derived cardiomyocytes? If yes, what method and machine is required to measure the contractility vs time? Any leads would be very much...

30 August 2021 1,260 5 View

Anyone tried making beating cardiac organoids from patient-derived iPSC lines ?

Has anyone here successfully made 3D cardiac organoid from human iPSC lines? I have tried multiple protocol to differentiate cells in 3D form using Wu protocol and kit based differentiation , but...

25 June 2021 9,386 3 View

IPSC derived cardiomyocytes clumping, how to reduce that?

Hi all, I have tried re-plating my HiPSC derived cardiomyocytes into another plate for functional analysis, but when I am passaging the cells form a whole clumpy layer that does not breakdown and...

23 June 2021 5,001 10 View

Has anyone tried replating HiPSC derived cardiomyocytes?

I want to measure contractility of the beating single cell and measure cell size. Cardiomyocytes die after reseeding.

04 February 2021 6,888 5 View

Is anyone experiencing total cell death after 48hrs of incubation with Wnt-C59 for HiPSC derived cardiomyocyte differentiation?

Hi All, I am starting the cardiac differentiation of my HiPSC lines using J.Wu lab's CDM3 based protocol. My cells remain healthy after CHIR99021 treatment of 6 uM and even 4uM for both 24 and 48...

09 October 2020 4,841 6 View