23 April 2024 1 1K Report

usgs has not given any scaling factor values to be applied for level 1 products. but it also doesn't make sense for satellite zenith angle to have values from 1-2768.

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To test chemical composition of different biomass types?

I want to test the chemical composition of Barley straw and Brown Algae biomass. By chemical composition, I mean testing Cellulose, Hemicellulose, and Lignin content in Barley Straw, while testing...

28 December 2023 8,873 4 View

In house Immunostaining protocol for cardiac organoids?

Hi researchers! Does anyone have a in-house immunostaining protocol to stain for organoids? I am guessing my antibody is not able to permeate well. If anyone has any in house protocol that works...

01 November 2021 5,818 7 View

Measuring contractility in hiPSC-CMs?

Hi Guys, Has anyone tried measuring contractility in hiPSC derived cardiomyocytes? If yes, what method and machine is required to measure the contractility vs time? Any leads would be very much...

29 August 2021 1,279 5 View

Anyone tried making beating cardiac organoids from patient-derived iPSC lines ?

Has anyone here successfully made 3D cardiac organoid from human iPSC lines? I have tried multiple protocol to differentiate cells in 3D form using Wu protocol and kit based differentiation , but...

24 June 2021 9,411 3 View

IPSC derived cardiomyocytes clumping, how to reduce that?

Hi all, I have tried re-plating my HiPSC derived cardiomyocytes into another plate for functional analysis, but when I am passaging the cells form a whole clumpy layer that does not breakdown and...

22 June 2021 5,030 10 View

Has anyone tried replating HiPSC derived cardiomyocytes?

I want to measure contractility of the beating single cell and measure cell size. Cardiomyocytes die after reseeding.

03 February 2021 6,910 5 View

Is anyone experiencing total cell death after 48hrs of incubation with Wnt-C59 for HiPSC derived cardiomyocyte differentiation?

Hi All, I am starting the cardiac differentiation of my HiPSC lines using J.Wu lab's CDM3 based protocol. My cells remain healthy after CHIR99021 treatment of 6 uM and even 4uM for both 24 and 48...

08 October 2020 4,860 6 View

Where to get the mathematical model of optimal power flow formulation used in Power World Software?

Where to get the mathematical model of optimal power flow formulation used in Power World Software? I am looking for the mathematical equation of loss in HVDC line used in Power World.

06 April 2020 4,270 0 View

In COD determination of municipal leachate open or closed reflux method will give the reliable result?

I am trying to find COD of municipal leachate using closed reflux method but results are not coming to the expectation. Following some journals i tried leachate before and after treatment COD...

05 April 2019 5,648 0 View

Mathematical model of compaction of polymers

How can I find mathematically a relationship between green density obtained for a polymer while pressure applied for compaction with hold time considered.

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