I am confused whether to subtract the absorbance values of samples with the absorbance values of field blanks or do I subtract those after obtaining the concentration?
It is common that if the sample OD at 405 nm was greater than the mean of the non infected controls plus 3 times their standard deviation, the sample is possibly infected with the pathogen of interest. However, sometimes the interpretation is not so easy and you will have to do PCR, IC- or RT-PCR according to your work. Bests, Masoud
Thanks, all of you. @Shen-An Hwang, field blanks here are treated like samples (in terms of method and transport) to capture background noises while doing field work. So, technically field blanks are also considered as samples right? Correct me if I'm wrong.