Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

How to estimate the error in the measured flow field by S-PIV?

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12 January 2021 8,271 3 View

Is it any standard method to preparation of active layer materials (blend donor & acceptor ) for organic bulk heterojunction solar cell ?

Hello friends , when I prepare blend donor and acceptor for active layer of organic solar cell I am take donor and acceptor in 1:1 ratio and concentration variation between 10mg/ml to 16mg/ml. I...

16 December 2020 3,876 4 View

How should I design electrodes for electrical stimulation on hydrogel ?

I would like to design custom setup for delivering electrical stimulation to cells cultured on electroconductive hydrogels. I am thinking to use standard tissue culture well plates (12 or 6 well...

26 October 2020 1,099 2 View

Can reduced particle size increase solubility ?

I'm trying to make Nanostructured lipid carriers (NLC) using ultrasonicator method for cancer meds that are hydrophobic and insoluble in water. I'm heating solid and liquid lipid phase and then...

21 October 2020 9,081 5 View

Please suggest any user friendly pipelines for the computation of Polygenic Risk Scores (PRS) on Microarray datasets?

I want to analyze the PRS scores for my microarray datasets, but want to use a platform which is a bit user friendly. Please suggest for any pipelines available.

06 October 2020 5,691 4 View

What is Zero air generator?

I want to purify NOx gas. Reported data shows that to achieve adsorption desorption equilibrium first we have to mix both gases.

05 October 2020 6,020 3 View

How to prepare nanoemulsion at home without a ultrasonic probe sonicator ?

I am trying to make a nano-emulsion to convert a cancer medicine into its more bioavailable form following guidance from this patent https://patents.google.com/patent/US9259391B2/en But I made...

17 September 2020 9,192 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...

03 February 2021 8,540 2 View

Are there any fast-acting, long-term fixatives compatible with FISH?

I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...

03 February 2021 7,659 7 View

How can I get access of UNAfold tool?

I am searching for tools to construct molecular beacons which shall be complementary to a given sequence. I don't want to design any primers. I found that UNAfold is one such tool which I could...

02 February 2021 9,065 5 View