Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

How to estimate the error in the measured flow field by S-PIV?

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12 January 2021 8,271 3 View

Is it any standard method to preparation of active layer materials (blend donor & acceptor ) for organic bulk heterojunction solar cell ?

Hello friends , when I prepare blend donor and acceptor for active layer of organic solar cell I am take donor and acceptor in 1:1 ratio and concentration variation between 10mg/ml to 16mg/ml. I...

16 December 2020 3,876 4 View

How should I design electrodes for electrical stimulation on hydrogel ?

I would like to design custom setup for delivering electrical stimulation to cells cultured on electroconductive hydrogels. I am thinking to use standard tissue culture well plates (12 or 6 well...

26 October 2020 1,099 2 View

Can reduced particle size increase solubility ?

I'm trying to make Nanostructured lipid carriers (NLC) using ultrasonicator method for cancer meds that are hydrophobic and insoluble in water. I'm heating solid and liquid lipid phase and then...

21 October 2020 9,081 5 View

Please suggest any user friendly pipelines for the computation of Polygenic Risk Scores (PRS) on Microarray datasets?

I want to analyze the PRS scores for my microarray datasets, but want to use a platform which is a bit user friendly. Please suggest for any pipelines available.

06 October 2020 5,691 4 View

What is Zero air generator?

I want to purify NOx gas. Reported data shows that to achieve adsorption desorption equilibrium first we have to mix both gases.

05 October 2020 6,020 3 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

Is this method mentioned for sporulation assessment reliable ?

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under...

05 January 2021 9,468 1 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Best way for knockout of gene in Bacillus genome?

Hi everyone! I would like to knockout a gene in the genome of Bacillus. To do that I tried to make a construct that has 1) a backbone, 2) a kanamycin resistance cassette, flanked by 3) the...

24 November 2020 489 1 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

How does the spores of Bacillus anthracis cause the anthrax disease and how can it be weaponized?

Anthrax is a disease caused by aerobic spore-forming Bacillus anthracis. This spore can resist environmental changes and resist biological extremes of heat, pH, chemicals, and other factors; it...

20 October 2020 4,658 8 View

Can I transform Bacillus Subtilus with ligation reaction directly?

Hi All, I want to transform bacillus subtilus with ligation reaction directly. Is that possible? To be more clear, I have a shuttling plasmid between bacillus and e.coli. I know usually we need...

14 October 2020 1,743 1 View