Hey everyone, has anyone ever performed immunohistochemical staining followed by histochemical staining on the same histological section (like a double labeling)?
Uma vez eu passei a lâmina de imuno pela bateria de HE sem querer e deu certo kk
I will do on two adjacent sections, on for histology and other for IHC. that will be better
I have done immunohistochemistry and then counterstained using either haematoxylin or methyl green. Works well and helps with working out location of IHC staining
Hello,
Yes, we routinely perform CD markers (ie CD3, CD68) with a PAS counterstain for renal biopsies. As long as you dont strip out the DAB or obscure it there should be no problem combining the procedures.
Many thanks,
Christopher Yeomans Could you please provide me with a step-by-step protocol on how you perform the double marking?
Péter Degrell Could you please provide me with a step-by-step protocol on how you perform the double marking?
I have performed histochemistry first, then I used this slide for immunohistochemistry. But, stain became so dark. It's better to do both procedures in separate slides.
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