How cell growth can impact some proteins expression?

Hello everyone, I would like to know how cell growth and proliferation can impact the expression of some proteins. In other terms if a fixed amount of cells was seeded in 12 well plate in Day0 and...

17 December 2020 8,392 1 View

How to make a thin polymer solution thicker?

We are fabricating a sensor in which we have to coat a polymer, which is sensitive to the measurement parameter on the Copper electrode. But the sensitive polymer solution is too thin and not...

11 August 2018 8,028 5 View

Can prosensus be applied for analysis of hyperspectral imaging process?

Can hyperspectral imaging results be analysed using prosensus?

16 January 2016 5,330 2 View

No title

23 December 2015 7,313 2 View

What is the boundary conditions in their vector forms for the coupled stress in fluid dynamics?

The normal stress and the boundary condition for the coupled stress is required?

01 January 1970 5,111 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

What's the difference between 0.1% triton-X100 and ice-cold methanol in the immunofluorescence assay for intracellular protein staining?

Hi, Guys, I noticed that researchers usually permeabilized the cells with 0.1% Triton-X100 or ice-cold methanol (90%) for an intracellular protein staining, it looks like that the two methods work...

15 February 2021 5,606 4 View

I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

11 February 2021 6,852 4 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

How to do immunofluorescence staining of the microtome sections of paraffin-embedded tissue ?

I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...

10 February 2021 3,113 3 View