Hello everyone,
I would like to know how cell growth and proliferation can impact the expression of some proteins. In other terms if a fixed amount of cells was seeded in 12 well plate in Day0 and then each day a Well was lysed. RNA extraction is performed for all samples and then reverse transcription then PCR. Knowing that for reverse transcription the same quantity of RNA is used for all samples, how can we explain the variation in the expression of the target ?
That is a complex topic. Cell state is dependent on multiple factors including: growth factors, nutritional needs, osmotic pressure, CO2 content, temperature,... which are dependent on your incubator and growth medium. Next, cell will slow down proliferation after they hit 70-80% confluence which will affect expression profile. I have noticed differences in expression within triplicates located on the same plate started from the same cell stock.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Plating