Hi 👋
i use image j to analyze my band of western blot previously i select band then analyze it by plotting curve and calculating area under curve .. recently when i analyze band this appear with no any curve draw .. what may be the reason ?? Iam new user for this application …
Probably, you're missing a step. There are lots of instructions out there, does any of them work? Google: "image j western blot"
Hi Duaa Adnan ,
If you want to avoid having to worry about the correct installation process for ImageJ, you can get a free trial of our Phoretix 1D software which is specifically designed to quantify Western Blot bands here - https://totallab.com/products/phoretix-1d/
It'll be much easier and quicker than using/troubleshooting ImageJ
Best wishes,
Steven
Hi there,
Could share the original picture and explain the error over it so we can try on it and help you?
That way should be easier to find a solution.
As to check that everything is ok in your images, perform a line selection over any lane or band in your western blot and then press Ctrl+K in ImageJ.
This command plots the intensity of the pixels under the line (see the attached picture), if everything is ok you'll get something similar to what I attach here.
Hope this helps,
Cheers,
J
Hello Steven Dodd
The installation process of ImageJ is quite straightforward mainly because there is no installation.
Luckily for us all, the internet is full of forums like this one (or the imageJ forum) in which you can ask for questions concerning ImageJ, and tons of qualified people from different fields of research will altruistically help you. That being superior to any "Help" manual in any commercial software.
As a plus, ImageJ does not have free trials. Is free.
Cheers,
J
Hi J. Ramirez-Franco
Image J is a fantastic sandbox tool for image analysis and as you say there's a plethora of people out there on forums etc. to help you with your analysis however it's not designed for a specific workflow beyond custom academic projects which have been built upon it's framework. Our software is designed to guide the user through a specific workflow in the quickest most reproducible way possible however it's completely possible to perform all the calculations and measurements required for analysis within Image J it just takes longer, especially for first time users.
Our free trial is for 2 weeks which is plenty of time for a user to evaluate the software and get results, even perform all their work if they only have a few gels/blots to analyse.
I present it as a quicker alternative for the uninitiated, I would argue that it's not truly "free" if it takes you 3-5x longer to perform the same functions as in another software as everyone's time has a cost associated. I appreciate this is more of a concern in a commercial environment rather than an academic one.
I like Image J, I think it's very good for what it is and I don't want to reduce this to some kind of "free" vs "commercial" debate, simply informing people that there are other options out there for them if they wish to give them a try.
Best wishes,
Steven
Here is a tutorial video on imagej western blot analysis
https://m.youtube.com/watch?v=u-u3G7JhIAo
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