I am developing protein subunit vaccines and DNA vaccines and I was hoping to find resources that would say how many weeks between doses is optimal, when are the best times to take bleeds, what kind of differences should I expect between the murine immune response and human response, typical dose amounts, typical levels of immune responses, and maybe what all organs are typically collected and how they are analyzed.
the majority of what your after can only really be known by trying the experiment with your specific vaccine as different vaccines will obviously work differently.
As a good starting point you could look at papers that use DNA subunit vaccines, ideally for whatever target you are targeting and see what they do.
this papers quite good for the differences between mice and humans immune responses https://www.jimmunol.org/content/172/5/2731
as an example of which organs to take for monitoring the immune system, previously, I’ve taken pbmc’s, tumours, spleens and lymph nodes before and analysed by FACS, ELISA and ELISPOT
Study the text book "Veterinary immunology bu Tyzer.
This text illustrate plan for vaccine production application ,in addition complete explain for vaccinology manufacture and applicatin.
To do the In vivo testing prior to start the dosage calculation, it should needed the appropriate adjuvant either complete or incomplete, based on the monographs from WHO vaccines and Immunobiological like TRS, Manual,
you will get the exact dosage calculation as per the approval of the regulatory norms.
It is essential to understand basic immunology before you take up your experimentation, whatever you do has to be theoretically correct!
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