Hello

See this links , may help you 

Good Luck

http://www.biocompare.com/pfu/110447/soids/8352/Antibodies/HA

http://www.antibodies-online.com/antibody/100176/anti-HA-Tag/

http://www.sigmaaldrich.com/catalog/product/sigma/sab4300603?lang=en®ion=SA

Thanks Saleh

Manish

Hey Manish

I can suggest one thing as I have tried this when I was getting much background. Take 3 third instar larvae, open them up and add them to the buffer in which you make your antibody dilution. I used TBST so I made 5%milk in TBST, used 1 ml of that and added the opened third instar larvae to that. Now if you are using 1:10,000 as your antibody concentration add 1 microlitre of that antibody directly to the 1ml solution containing opened larvae. Keep this at 4degC o/n with constant vortexing. Next day do a quick spin and suck the solution and use that for your immunoblot procedure. Hope this helps.

Good luck.

Hi Manish,

I have had a lot of success with Rb a-HA mAb from Cell Signaling (link below). Otherwise, the pre absorption protocol Mayank suggested would be a suitable alternative-- although, I preabsorb using embyros.

http://www.cellsignal.com/product/productDetail.jsp?productId=3724

Cheers