Why is my primer amplifying multiple products despite specificity, yet resolved with SNaPshot?

I have several COMT SNPs which I designed primers for using BatchPrimer3. I did all my checks and did a BLAST for all primers to ensure it binds to the correct region. All primers have worked...

14 September 2020 4,143 4 View

ABC elite kit - should I agitate while incubating tissue with ABC?

I inherited a staining protocol from another lab. It specifically says not to swirl the ABC mixture, but instead to let it sit for 30 minutes before adding the tissue. It does not specify if the...

26 January 2020 5,868 2 View

Results on Parameter-Dependent Pareto Front?

Dear community, I would like to request some references related to results on parameter-dependent Pareto Front, if there are any. I am interested in studying the behavior of the Pareto Front with...

08 May 2019 9,669 3 View

How do I quantify cells and fibers in tissues used in immunohistochemistry?

Hi all, I would need to compare the number of cells and fibers in different part of a tissue which was used in fluorescent immunohistochemistry. However, I am not sure how to proceed. I could use...

21 March 2019 8,043 3 View

Plasmid Problems: Is it possible to use RE to cut a section out of a plasmid & immediately amplify it?

I have a 1.6kb insert that has been cloned into a plasmid. I would like to cut a section out of this plasmid that is flanked by restriction sites for ECOR1. Would it be possible to digest the...

11 February 2019 2,848 7 View

Convert a 3D direction vector to yaw and pitch?

I am trying to convert 3D direction vectors (see example) to yaw and pitch (apparently you can't get roll from the vectors). My ultimate goal is to determine the Euler angles from my answers....

09 September 2018 1,472 1 View

Using the gromacs md run -rerun command, is it possible to generate bonded terms?

Hi, I ran some MD simulations in GROMACS and am trying to get a sense of the changes in potential energy of the active site between conditions (with and without inhibitor present). I have...

25 April 2018 7,688 5 View

Running MD simulations on small molecules and generating parameters ?

I am generating small molecule parameters using PRODRG and ABT. Why exactly does PRODRG take less time then ABT? I want to know this because I am trying to decide if it is worth it to wait for ABT...

03 April 2018 5,705 2 View

What Big Data solution use for deep learning on clinical data purpose ?

Hello everyone, I would like to perform machine learning and deep learning in a near futur on clinical data, coming from different sources and technologies (Omics data, Imaging data, and so...

23 August 2017 3,551 5 View

NLP : Short text classification/categorization

Hello everyones, I'm looking for a way to classify really short pieces of text (max 18 words) into 9 different classes. As a learning set, I've got a dataframe with each sentence (one column)...

12 July 2017 2,965 10 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View