Why is my primer amplifying multiple products despite specificity, yet resolved with SNaPshot?

I have several COMT SNPs which I designed primers for using BatchPrimer3. I did all my checks and did a BLAST for all primers to ensure it binds to the correct region. All primers have worked...

14 September 2020 4,143 4 View

ABC elite kit - should I agitate while incubating tissue with ABC?

I inherited a staining protocol from another lab. It specifically says not to swirl the ABC mixture, but instead to let it sit for 30 minutes before adding the tissue. It does not specify if the...

26 January 2020 5,868 2 View

Plasmid Problems: Is it possible to use RE to cut a section out of a plasmid & immediately amplify it?

I have a 1.6kb insert that has been cloned into a plasmid. I would like to cut a section out of this plasmid that is flanked by restriction sites for ECOR1. Would it be possible to digest the...

11 February 2019 2,848 7 View

Convert a 3D direction vector to yaw and pitch?

I am trying to convert 3D direction vectors (see example) to yaw and pitch (apparently you can't get roll from the vectors). My ultimate goal is to determine the Euler angles from my answers....

09 September 2018 1,472 1 View

Using the gromacs md run -rerun command, is it possible to generate bonded terms?

Hi, I ran some MD simulations in GROMACS and am trying to get a sense of the changes in potential energy of the active site between conditions (with and without inhibitor present). I have...

25 April 2018 7,688 5 View

Running MD simulations on small molecules and generating parameters ?

I am generating small molecule parameters using PRODRG and ABT. Why exactly does PRODRG take less time then ABT? I want to know this because I am trying to decide if it is worth it to wait for ABT...

03 April 2018 5,705 2 View

Can you detect secreted ATP by luminescence if your supernatans contains FBS?

26 April 2017 3,760 5 View

A question on Atlas ti for data analysis?

I asked this question last year already and someone responded ... I would really like to know how to go about Atlas ti for data analysis. Unfortunately I do not have resources to attend a...

23 March 2017 7,421 3 View

Is there a method to score therapist interventions or attitudes in CBT groups?

I'm looking for a scoring system that differentiates therapists interventions in CBT groups. I want to know what kind of attitude, interpersonal style works in treating batterers. The specific...

08 September 2016 6,613 1 View

IFN-g staining: Does someone know a good IFNg antibody to stain human tissue?

09 February 2016 4,411 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

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25 February 2021 5,713 1 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

How can we determine the band gap of a composite material that is made up of a composition of direct and indirect band gap semiconductor materials?

I have a direct band gap material and an indirect band gap material. If I made a composite of both through physical mixing. Then how can I determine the band gap of such a material as the Tauc...

17 February 2021 8,104 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View