if the gene of my interest is knockout in cell lines in CRISPR and the gene effect (Chronos)was zero or positive 0.1 or 0.2 in this case is the cell survive or die? please need an answer.
https://forum.depmap.org/t/crispr-ko-the-gene-of-interests/1431?u=maryam
Christopher Lange
It is often the case that if a gene is KO'd there is another gene or pathway to accomplish the same thing. Therefore, no apparent effect of the KO. Look for the absence of gene product in the KO'd cells to compare effectiveness of the KO. You may be able to find an alternative gene with enhanced expression using individual cell RNAseq to find possible backup pathway(s).
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