I've read that template-switching can create duplications, but those duplications are inverted (aka TIDs - tandem inverted duplications).
Can template-switching result in a non-inverted duplication as well? If so please pass reference to me. Thanks!
The inversion occurs due to palindromic sequences which are usually at stem loop secondary structures. Else there is microhomology in the opposite orientation.
You may find this reference useful:
(Article Mechanisms of change in gene copy number
)
Robert Adolf Brinzer
Thanks! The palindromic sequence makes sense for the inversions.
No, the resultant duplication is not always inverted during template-switching in DNA replication. It can be either in the same orientation as the original sequence (tandem duplication) or inverted (inverted duplication) depending on the specific mechanism and circumstances of the replication error.@Ryan Gourlie
Atta Ur Rahman thank you for you comment, can you explain which circumstances lead to non-inverted and which to inverted?
In certain DNA replication errors, particularly during template-switching events like strand slippage or misalignment, the resulting duplication can indeed be either in the same orientation (tandem duplication) or inverted (inverted duplication) relative to the original sequence. This can occur under specific circumstances and mechanisms during DNA replication.
In template-switching, which is often associated with replication errors like slipped-strand mispairing, the DNA polymerase temporarily dissociates from the template strand and switches to a nearby template strand before continuing replication. The orientation of the newly synthesised DNA strand and the template strand to which it switches can determine whether the resulting duplication is in the same or inverted orientation. Here's a basic explanation:
The specific circumstances and outcomes of template-switching events can vary, and they are influenced by factors such as the length and sequence of the repetitive elements in the DNA, the timing of the switch, and the precise mechanisms involved in DNA replication. These events can lead to genetic rearrangements and contribute to the diversity of genomic structures, which can have implications in terms of genetic variation and evolution.
Ryan Gourlie
Not necessarily. Template-switching during DNA replication can lead to duplications, but whether they are inverted or not depends on the specific mechanisms involved. It's not a strict rule.
Hi please read this is I think it will helpful for you see link below:
https://doi.org/10.7554/eLife.41697
Thank you
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