I want to do some gene expression studies in my target insect. My sequence 85% identity from NCBI.how check specificity of my primers?.........Thanks advance.......
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My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...
22 February 2021 2,965 4 View
The reaction between N-S bidentate/ONS tridendate ligand (TSC) and cis[RuCl2(DMSO)4] in ethanol by reflux is complete as seen from TLC chromatography. But removing the excess solvent through...
27 December 2020 6,630 1 View
The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...
09 December 2020 4,340 3 View
09 December 2020 9,960 3 View
I am running MD simulations for holoenzyme in Gromacs. The protein has four chains like A, B, C, D. After MD simulations, All the chains are showing as X chain in VMD and even in the PDB file....
12 November 2020 6,228 1 View
In one of my studies, I need to assess the sample size. The study aims to predict the choice of participants with a continuous predictor and a categorical predictor (control vs. experimental)....
26 October 2020 8,631 6 View
I am electroplating Ni on a stainless steel for abrasive application. At temperature and time can I anneal so that Ni thermally diffuses into stainless steel? My intention is to improve the...
20 October 2020 7,092 3 View
While i try to import the data from excel files to NLogit 4, only 18-19 variables being imported and the rest 6-7 variables is missing in the imported list. I have tried in changing the memory...
12 November 2019 9,760 1 View
13 June 2018 5,388 3 View
Hi, I am currently working on ISSR primers for my algal samples. When I did PCR for my samples along with some marine algal specimens, I could observe bands for the marine specimens and a smear...
15 May 2018 877 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View