I want to do some gene expression studies in my target insect. My sequence 85% identity from NCBI.how check specificity of my primers?.........Thanks advance.......
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for example, in the range of 3.1 ppm, instead of 4H we got 1.8 protons
12 March 2024 9,931 0 View
I am writing a paper on employee instruction program using Augmented Reality. I am gathering information about hotels using AR to provide training to their employees for improving the performance....
18 February 2024 6,288 2 View
I want the procedure for Nitric oxide assay in plant sample.. Please kindly help in this regard at the earliest .. send an email to [email protected].
30 December 2023 9,753 1 View
The synthesized nanocomposite is in powder form and can be diluted in water. How to know the suitable electrolyte and electrode for the CV experiments. Also, please provide me the equation to...
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Please guide me on essential characterization techniques to prove the formation of graphene quantum dots. For example, I have TEM, SEM, FTIR and XRD data for the synthesized GQDs. I want to know...
28 July 2023 3,547 3 View
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11 July 2023 3,307 1 View
How to calculate sample size for multi-arm (three arm) RCT?
21 April 2023 4,910 1 View
In a CHB based multilevel inverter how to find the RMS output voltage of each cell when modulated by Sinusoidal pulse width modulation?
09 April 2023 1,975 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View