Can someone provide a simple method for isolating Circulating Tumor Cells (CTCs) from mice blood? I need a protocol that doesn't require antibodies and can be done manually.
I would greatly appreciate any help provided.
Thank you in advance!
There are many commercially available size-based filters you can try -- for example, screencell (or something like it).
You could also try density gradient centrifugation with something like histopaque followed by filtration to get a more CTC-targeted (in the sense of size) approach.
Shameless plug for some of my work -- here's a (somewhat recent) review paper that might interest you.
Article Cell Separations and Sorting
CTCs are "rare" ... but also kind of not -- tumors release about 3 million cells per gram of tumor per day. Yet many current technology will "only" isolate
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