If I double the length size of my chromatography column, what would be the resolution fold increment?

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Can glycerol pass through a 3KDa dialysis membrane?

I am dialysing a 40KDa protein in a 3Kda dialysis membrane. The buffer that my protein is in has some glycerol remaining. So I want to make sure the glycerol is not there anymore. I ran some...

03 April 2016 5,525 5 View

Amber software experts (molecular dynamics) interested in participating in a small research project?

Please Inbox for more information

07 August 2015 9,483 0 View

How to find intramolecular interactions within a protein?

I need to find intra-molecular interactions within a protein like hydrophobic, electrostatic, and van der Waals interactions. Do you know a program that can find this interactions and how to do...

01 February 2015 5,894 9 View

How can I save a mutagenesis done in PYMOL as PDB file?

I am doing point mutations in PYMOL and I want to run molecular dynamics (MD) to see the effect of the mutation in the stability of my protein. In order to use the software Amber that does the MD...

31 December 2014 10,142 8 View

Any suggestions on how to keep my protein soluble without using too much urea?

I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...

08 September 2013 685 11 View

What is the most aggressive type of breast cancer?

Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.

04 May 2013 8,518 7 View

What is the distance between the rings in a ring stacking interaction in proteins?

I would like to know the distance between the rings in a ring stacking interaction in proteins.

03 April 2013 6,321 4 View

Is it ok if I do miniprep of my DNA of interest from an expression cell line like E. coli M15 culture?

I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...

11 December 2012 4,963 7 View

Commercial available protein that does not contain disulfide bonds.

Does anyone know a commercial available and inexpensive protein that does not contain disulfide bonds?

10 November 2012 6,528 6 View

Protein no longer expressed

I successfully expressed my protein a while ago and suddenly it just stop expressing. I can't find out what the problem is, the DNA is inserted in a pQE30Xa plasmid and it has a very hydrophobic...

07 August 2012 6,801 13 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View