Can glycerol pass through a 3KDa dialysis membrane?

03 April 2016 5,428 5 View

Amber software experts (molecular dynamics) interested in participating in a small research project?

07 August 2015 9,434 0 View

How to find intramolecular interactions within a protein?

01 February 2015 5,825 9 View

How can I save a mutagenesis done in PYMOL as PDB file?

31 December 2014 9,958 8 View

Any suggestions on how to keep my protein soluble without using too much urea?

I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...

08 September 2013 632 11 View

What is the most aggressive type of breast cancer?

Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.

04 May 2013 8,441 7 View

What is the distance between the rings in a ring stacking interaction in proteins?

I would like to know the distance between the rings in a ring stacking interaction in proteins.

03 April 2013 6,159 4 View

Is it ok if I do miniprep of my DNA of interest from an expression cell line like E. coli M15 culture?

I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...

11 December 2012 4,907 7 View

Commercial available protein that does not contain disulfide bonds.

Does anyone know a commercial available and inexpensive protein that does not contain disulfide bonds?

10 November 2012 6,473 6 View

Protein no longer expressed

I successfully expressed my protein a while ago and suddenly it just stop expressing. I can't find out what the problem is, the DNA is inserted in a pQE30Xa plasmid and it has a very hydrophobic...

07 August 2012 6,744 13 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View